| Library |
| Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
| Calyx |
Total RNA was extracted from flower buds, fully-opened flowers, pistils, petals, calyxes, and roots using the Plant Total RNA Isolation Kit (Biomarker, Bejing, China) and DNA contamination was eliminated by RNase-Free DNase I kit (Takara, Dalian, China). RNA was evaluated using the same method as with DNA. A fluorometer integrated and qualified RNA was used for cDNA library construction after testing with NanoDrop spectrophotometer and a Qubit 2.0. After the PCR products were purified, the library was assessed on the Agilent Bioanalyzer 2100. Assessed libraries were sequenced on Illumina HiSeq X Ten platform (Illumina, San Diego, CA, USA) according to the manufacturer’s instructions. |
RNA-Seq |
TRANSCRIPTOMIC |
unspecified |
PAIRED
|
|
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