Experiment information
Accession CRX479202
Organism Armillaria
Title A37_3
BioProject PRJCA005934
BioSample SAMC727154
Platform DNBSEQ-T7
Library name Construction protocol Strategy Source Selection Layout
Total RNA was isolated from samples using the Trizol reagent (Invitrogen, USA) according to the manufacturer’s instructions. RNA quality and quantity were determined with a spectrophotometer (IMPLEN, Germany) and an Agilent 2100 Bioanalyzer (Agilent Technologies, USA). RNA-seq was performed at the Annoroad Company (Beijing, China). The RNA-Seq library was constructed using the NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (NEB, USA). The mRNA was purified from total RNA using poly-T oligo-attached magnetic beads. The cleaved RNA fragments were transcribed into first-strand cDNA using reverse transcriptase and subsequently second-strand cDNA synthesis was performed using DNA polymerase I and RNase H. The fragments were ligated to sequencing adaptors and the library preparations were sequenced on an Illumina HiSeq 4000 platform and 150 bp paired-end reads were generated. RNA-Seq TRANSCRIPTOMIC RANDOM PAIRED
Processing Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Release date2023-03-19
Run accession Run data file information
File nameFile size (MB)
CRR539606 A37_5_R1.fq.gz
SubmitterJianqiang Wu (wujianqiang@mail.kib.ac.cn)
OrganizationChinese Academy of Sciences
Date submitted2022-08-03
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