| Accession | CRX499804 |
| Organism | Mus musculus |
| Title | Klf4-WT-RIP-seq-Rep3 |
| BioProject | PRJCA011575 |
| BioSample | SAMC867515 |
| Platform | Illumina HiSeq 4000 |
| Library |
| Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
RIP library was constructed with EZ-Magna Nuclear RIP™ (Cross-Linked) Nuclear RNA-Binding Protein Immunoprecipitation KitMillipore Kit (Millipore), using antibodies of Klf4 (R&D) and FLAG(Sigma). Libraries were then constructed and indexed with NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina® (NEB). The size and concentration of library was examined with the DNA bioanalyzer. |
RIP-Seq |
TRANSCRIPTOMIC |
PCR |
PAIRED
|
|
| Processing |
Planned read length (bp) for mate 1: 100
Planned read length (bp) for mate 2: 100
|
| Release date | 2022-09-01 |
|---|
| Run |
| Run accession |
Run data file information |
| File name | File size (MB) |
|---|
| CRR563753 |
CRR563753_f1.fastq.gz
CRR563753_r2.fastq.gz
|
348.16
407.49
|
|
| Submitter | Wenbin Wang (sciencedb@gibh.ac.cn) |
|---|
| Organization | Nankai University |
|---|
| Date submitted | 2022-08-31 |
|---|
