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Experiment information
Accession CRX503404
Organism Eospalax fontanierii baileyi
Title BSA_2
BioProject PRJCA010603
BioSample SAMC874548
Platform Illumina NovaSeq 6000
Library
Library name Construction protocol Strategy Source Selection Layout
Total RNA was used as input material for the RNA sample preparations. Briefly, mRNA was purified from total RNA by using poly-T oligo-attached magnetic beads. Fragmentation was carried out using divalent cations under elevated temperature in First Strand Synthesis Reaction Buffer(5X). First strand cDNA was synthesized using random hexamer primer and M-MuLV Reverse Transcriptase, then use RNaseH to degrade the RNA.Second strand cDNA synthesis was subsequently performed using DNA Polymerase I and dNTP. RNA-Seq TRANSCRIPTOMIC PCR PAIRED
Processing Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Release date2022-09-08
Run
Run accession Run data file information
File nameFile size (MB)
CRR567498 CRR567498_f1.fq.gz
CRR567498_r2.fq.gz
1,389.38
1,435.28
SubmitterBaohui Yao (735310030@qq.com)
OrganizationGansu Agricultural University
Date submitted2022-09-08