| Accession | CRX503409 |
| Organism | Eospalax fontanierii baileyi |
| Title | NBS_1 |
| BioProject | PRJCA010603 |
| BioSample | SAMC874553 |
| Platform | Illumina NovaSeq 6000 |
| Library |
| Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
Total RNA was used as input material for the RNA sample preparations. Briefly, mRNA was purified from total RNA by using poly-T oligo-attached magnetic beads. Fragmentation was carried out using divalent cations under elevated temperature in First Strand Synthesis Reaction Buffer(5X). First strand cDNA was synthesized using random hexamer primer and M-MuLV Reverse Transcriptase, then use RNaseH to degrade the RNA.Second strand cDNA synthesis was subsequently performed using DNA Polymerase I and dNTP. |
RNA-Seq |
TRANSCRIPTOMIC |
PCR |
PAIRED
|
|
| Processing |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
|
| Release date | 2022-09-08 |
|---|
| Run |
| Run accession |
Run data file information |
| File name | File size (MB) |
|---|
| CRR567503 |
CRR567503_f1.fq.gz
CRR567503_r2.fq.gz
|
1,326.31
1,373.33
|
|
| Submitter | Baohui Yao (735310030@qq.com) |
|---|
| Organization | Gansu Agricultural University |
|---|
| Date submitted | 2022-09-08 |
|---|
