| 实验编号 | CRX503411 |
| 物种名称 | Eospalax fontanierii baileyi |
| 标题 | NBS_3 |
| 项目编号 | PRJCA010603 |
| 样本编号 | SAMC874555 |
| 测序平台 | Illumina NovaSeq 6000 |
| 建库信息 |
| 文库名称 |
文库构建方法 |
建库策略 |
样品来源 |
实验选择 |
文库布局 |
|
Total RNA was used as input material for the RNA sample preparations. Briefly, mRNA was purified from total RNA by using poly-T oligo-attached magnetic beads. Fragmentation was carried out using divalent cations under elevated temperature in First Strand Synthesis Reaction Buffer(5X). First strand cDNA was synthesized using random hexamer primer and M-MuLV Reverse Transcriptase, then use RNaseH to degrade the RNA.Second strand cDNA synthesis was subsequently performed using DNA Polymerase I and dNTP. |
RNA-Seq |
TRANSCRIPTOMIC |
PCR |
PAIRED
|
|
| 处理信息 |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
|
| 发布日期 | 2022-09-08 |
|---|
| 测序反应 |
| Run编号 |
Run序列文件信息 |
| File name | File size (MB) |
|---|
| CRR567505 |
CRR567505_f1.fq.gz
CRR567505_r2.fq.gz
|
1,398.45
1,447.75
|
|
| 提交者 | Baohui Yao (735310030@qq.com) |
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| 所属单位 | Gansu Agricultural University |
|---|
| 提交日期 | 2022-09-08 |
|---|
