Accession | CRX508618 |
Organism | Mus musculus |
Title | scATAC_2W_2 |
BioProject | PRJCA011963 |
BioSample | SAMC898737 |
Platform | Illumina NovaSeq 6000 |
Library |
Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
The prostates of TPPRC mice were dissected and minced with scissors. The dissociated prostate cells were resuspended in Red Blood Cell Lysis Solution (Miltenyi Biotec, no. 130-094-183) and incubated at room temperature for 2 min. Dead cells were removed with Dead Cell Removal Kit (Miltenyi Biotec, no. 130-090-101), and the cells were counted via the Countess II FL Automated Cell Counter (Thermo Fisher Scientific). Cells were lysed on ice for 5 min. Isolated nuclei from T2PPRC mouse prostates cells were transposed and loaded onto the Chromium Next GEM Chip J (10X Genomics). Then, the chip with prostate cells was loaded on a Chromium Controller. The library was constructed according to the manufacturer’s instructions. |
OTHER |
GENOMIC SINGLE CELL |
other |
PAIRED
|
|
Processing |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Insert size (bp): 400
|
Release date | 2022-10-04 |
Run |
Run accession |
Run data file information |
File name | File size (MB) |
CRR572830 |
CRR572830.tar.gz
|
67,905.17
|
|
Submitter | Fei Li (lifei6@sibcb.ac.cn) |
Organization | Center for Excellence in Molecular Cell Science/Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences |
Date submitted | 2022-09-23 |