| Accession | CRX508621 |
| Organism | Mus musculus |
| Title | scATAC_4_5M_1 |
| BioProject | PRJCA011963 |
| BioSample | SAMC898740 |
| Platform | Illumina NovaSeq 6000 |
| Library |
| Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
The prostates of TPPRC mice were dissected and minced with scissors. The dissociated prostate cells were resuspended in Red Blood Cell Lysis Solution (Miltenyi Biotec, no. 130-094-183) and incubated at room temperature for 2 min. Dead cells were removed with Dead Cell Removal Kit (Miltenyi Biotec, no. 130-090-101), and the cells were counted via the Countess II FL Automated Cell Counter (Thermo Fisher Scientific). Cells were lysed on ice for 5 min. Isolated nuclei from T2PPRC mouse prostates cells were transposed and loaded onto the Chromium Next GEM Chip J (10X Genomics). Then, the chip with prostate cells was loaded on a Chromium Controller. The library was constructed according to the manufacturer’s instructions. |
OTHER |
GENOMIC SINGLE CELL |
other |
PAIRED
|
|
| Processing |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Insert size (bp): 400
|
| Release date | 2022-10-04 |
|---|
| Run |
| Run accession |
Run data file information |
| File name | File size (MB) |
|---|
| CRR572833 |
CRR572833.tar.gz
|
65,561.33
|
|
| Submitter | Fei Li (lifei6@sibcb.ac.cn) |
|---|
| Organization | Center for Excellence in Molecular Cell Science/Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences |
|---|
| Date submitted | 2022-09-23 |
|---|
