| Accession | CRX508627 |
| Organism | Mus musculus |
| Title | ATAC_late |
| BioProject | PRJCA011963 |
| BioSample | SAMC898746 |
| Platform | Illumina NovaSeq 6000 |
| Library |
| Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
n brief, cells were collected and washed once with PBS. The cells were then lysed in ice-cold lysis buffer (10 mM Tris-HCl, pH 7.4; 10 mM NaCl; 3 mM MgCl2; 0.1% NP-40; 0.1% Tween 20; and 0.01% digitonin) for 3 min on ice. Immediately after lysis, nuclei were washed with 1 mL of wash buffer (10 mM Tris-HCl, pH 7.4; 10 mM NaCl; 3 mM MgCl2; and 0.1% Tween 20) and then centrifuged at 500 g for 10 min at 4 °C. To prepare sequencing libraries, a TruePrep DNA Library Prep Kit V2 for Illumina (Vazyme, TD501) was utilized for the following steps. |
ATAC-seq |
GENOMIC |
size fractionation |
PAIRED
|
|
| Processing |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Insert size (bp): 400
|
| Release date | 2022-10-04 |
|---|
| Run |
| Run accession |
Run data file information |
| File name | File size (MB) |
|---|
| CRR572839 |
CRR572839_f1.fastq.gz
CRR572839_r2.fastq.gz
|
3,840.08
3,726.84
|
|
| Submitter | Fei Li (lifei6@sibcb.ac.cn) |
|---|
| Organization | Center for Excellence in Molecular Cell Science/Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences |
|---|
| Date submitted | 2022-09-23 |
|---|
