| 实验编号 | CRX509754 |
| 物种名称 | human gut metagenome |
| 标题 | P_8_faeces_mrs_12h |
| 项目编号 | PRJCA012199 |
| 样本编号 | SAMC909551 |
| 测序平台 | MGISEQ-2000RS |
| 建库信息 |
| 文库名称 |
文库构建方法 |
建库策略 |
样品来源 |
实验选择 |
文库布局 |
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DNA was extracted from the faeces or microbiota using a QIAamp MagAttract HMW DNA Kit, we constructed metagenomics co-barcoding sequencing system, which named as MECOS. Next, we would complete the MECOS library construction use high-molecular-weight DNA from faeces. To achieve more bacteria, we combined the culturing procedures for human microbiota and MECOS which named as cMECOS. The sequencing libraries of MECOS and cMECOS were constructed based on customized process, which mainly included the following steps: 1) Transposon insertion; 2) Co-barcoding of long fragment; 3) Ligation and PCR; 57) DNB generation and sequecing. |
OTHER |
METAGENOMIC |
other |
PAIRED
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| 处理信息 |
Planned read length (bp) for mate 1: 100
Planned read length (bp) for mate 2: 100
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| 发布日期 | 2023-03-28 |
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| 测序反应 |
| Run编号 |
Run序列文件信息 |
| File name | File size (MB) |
|---|
| CRR573994 |
CRR573994_f1.fq.gz
CRR573994_r2.fq.gz
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1,478.78
1,526.74
|
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| 提交者 | Chen Chen (chenchen1@ccmu.edu.cn) |
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| 所属单位 | Beijing Shijitan Hospital, Capital Medical University |
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| 提交日期 | 2022-09-28 |
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