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Experiment information
Accession CRX513476
Organism Oryza sativa
Title B001_WT
BioProject PRJCA012425
BioSample SAMC917311
Platform Illumina NovaSeq 6000
Library
Library name Construction protocol Strategy Source Selection Layout
In order to discover the DEGs on mRNA level in Rice Seeds (3DAPs) under 2021 field condition; construction of the NAC TFs regulated network. The total RNA are extracted from seeds (3DAP) in Nac mutants vs WT,and reverse-transferred into cDNA, then through amplification by PCR ,and constructed a library with 300 bp±50bp cDNA fragments. The library would be sequenced by illumina Novaseq™ 6000. RNA-Seq TRANSCRIPTOMIC PCR PAIRED
Processing Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Insert size (bp): 350
Release date2023-08-01
Run
Run accession Run data file information
File nameFile size (MB)
CRR578231 CRR578231_f1.fq.gz
CRR578231_r2.fq.gz
1,135.06
1,162.57
Submitteryan mei (82101181069@caas.cn)
Organizationhttps://cnrri.caas.cn/index.htm
Date submitted2022-10-11
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