| 实验编号 | CRX519876 |
| 物种名称 | Rhinolophus thomasi |
| 外部数据库编号 |
SCIENCE DATA BANK: |
| 标题 | RNA-seq of Rhinolophus_thomasi-Brain-1: Biological1 |
| 项目编号 | PRJCA012397 |
| 样本编号 | SAMC916840 |
| 测序平台 | Illumina NovaSeq 6000 |
| 建库信息 |
| 文库名称 |
文库构建方法 |
建库策略 |
样品来源 |
实验选择 |
文库布局 |
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Total RNA was isolated from frozen tissue using TRIzol® Reagent (Invitrogen). To protect RNA as much as possible during homogenization, we first added 0.2 mL TRIzol® Reagent directly to a tube containing 100 mg of frozen tissue and homogenized using a motorized homogenizer. After homogenization, we added another 0.8 mL of TRIzol® to the tube. The resulting lysate was phase separated with 0.2 ml chloroform and total RNA precipitated with 0.5 ml isopropanol. The RNA was washed twice with 1 ml 75% ethanol and resuspended in DEPC treated ddH2O. The resuspended RNA was assessed for quality (260/280 nm absorbance ratio) and integrity (formaldehyde agarose gel electrophoresis). The sequencing libraries were prepared using the NEBNext Ultra RNA Library Prep Kit for Illumina (NEB, USA), and the transcriptome libraries were sequenced on an Illumina NovaSeq 6000 system (Novogene Co. Ltd) with paired-end reads of 150 bp. |
RNA-Seq |
TRANSCRIPTOMIC |
cDNA |
PAIRED
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|
| 处理信息 |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
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| 发布日期 | 2022-12-07 |
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| 测序反应 |
| Run编号 |
Run序列文件信息 |
| File name | File size (MB) |
|---|
| CRR584669 |
CRR584669_f1.fq.gz
CRR584669_r2.fq.gz
|
916.08
960.82
|
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| 提交者 | Xuming Zhou (zhouxuming@ioz.ac.cn) |
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| 所属单位 | Institute of Zoology, Chinese Academy of Sciences |
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| 提交日期 | 2022-10-12 |
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