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Experiment information
Accession CRX520124
Organism Lyophyllum decastes
Title LRG-d1-1-Illumina
BioProject PRJCA012490
BioSample SAMC918121
Platform Illumina NovaSeq 6000
Library
Library name Construction protocol Strategy Source Selection Layout
luronggu_1_Illumina_I The obtained monokaryon mycelia were cultured on 20 Potato Dextrose Agar (PDA) plates covered with cellophane at 25°C in darkness for 16 days. These mycelia were then collected, frozen in liquid nitrogen, and used for genome sequencing and chromosome-level genome construction. The genome of this fungus was extracted using the NucleoBond HMW DNA kit (Macherey-Nagel, Düren, Germany). The concentration and quality of the DNA were analyzed using Thermal Nanodrop One (USA, CA) and Agilent 2100 Bioanalyzer System (USA, CA). The genome was then sequenced using Illumina NovaSeq 6000 (paired-end, 2 × 150 bp, insert size, 400 bp) WGS GENOMIC unspecified PAIRED
Processing Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Release date2022-10-17
Run
Run accession Run data file information
File nameFile size (MB)
CRR584946 CRR584946_f1.fq.gz
CRR584946_r2.fq.gz
1,305.95
1,331.03
SubmitterHao Yu (yuhaosunshine@163.com)
OrganizationQingdao Agricultural University
Date submitted2022-10-14
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Experiments(2)