Library |
Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
luronggu_1_Nanopore_I |
The obtained monokaryon mycelia were cultured on 20 Potato Dextrose Agar (PDA) plates covered with cellophane at 25°C in darkness for 16 days. These mycelia were then collected, frozen in liquid nitrogen, and used for genome sequencing and chromosome-level genome construction. The genome of this fungus was extracted using the NucleoBond HMW DNA kit (Macherey-Nagel, Düren, Germany). The concentration and quality of the DNA were analyzed using Thermal Nanodrop One (USA, CA) and Agilent 2100 Bioanalyzer System (USA, CA). The genome was then sequenced using Illumina Oxford Nanopore platform. |
WGS |
GENOMIC |
unspecified |
SINGLE
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