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Experiment information
Accession CRX520138
Organism Lyophyllum decastes
Title LRG-d1-5-Nanopore
BioProject PRJCA012490
BioSample SAMC918124
Platform OXFORD_NANOPORE PromethION
Library
Library name Construction protocol Strategy Source Selection Layout
luronggu_5_Nanopore_I The obtained monokaryon mycelia were cultured on 20 Potato Dextrose Agar (PDA) plates covered with cellophane at 25°C in darkness for 16 days. These mycelia were then collected, frozen in liquid nitrogen, and used for genome sequencing and chromosome-level genome construction. The genome of this fungus was extracted using the NucleoBond HMW DNA kit (Macherey-Nagel, Düren, Germany). The concentration and quality of the DNA were analyzed using Thermal Nanodrop One (USA, CA) and Agilent 2100 Bioanalyzer System (USA, CA). The genome was then sequenced using Illumina Oxford Nanopore platform. WGS GENOMIC unspecified SINGLE
Processing
Release date2022-10-15
Run
Run accession Run data file information
File nameFile size (MB)
CRR584960 CRR584960.fq.gz 8,885.61
SubmitterHao Yu (yuhaosunshine@163.com)
OrganizationQingdao Agricultural University
Date submitted2022-10-14
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