| Accession | CRX527948 |
| Organism | Pyrus pyrifolia |
| Title | The RNA-seq data of the fruits of 'Qiushui' at 110 days after blossom replication 1 |
| BioProject | PRJCA012773 |
| BioSample | SAMC959351 |
| Platform | Illumina HiSeq 4000 |
| Library |
| Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
The cDNA libraries were constructed with approximately 5 ug of RNA for each sample using the NEBNext Ultra RNA Library Prep Kit according to the manufacturer's instructions and index codes were added to attribute sequences to each sample. To select cDNA fragments of preferentially 250-300 bp in length, the library fragments were purified using the AMPure XP System. Then PCR was performed by Universal PCR primers, Index (X) Primer, and cDNA. The PCR products were purified and library quality was assessed on the Agilent Bioanalyzer 2100 system. Each library was used for Paired-End sequencing using Illumina HiSeq4000. |
RNA-Seq |
GENOMIC |
RANDOM PCR |
PAIRED
|
|
| Processing |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
|
| Release date | 2023-02-16 |
|---|
| Run |
| Run accession |
Run data file information |
| File name | File size (MB) |
|---|
| CRR592912 |
CRR592912_f1.fastq.gz
CRR592912_r2.fastq.gz
|
1,657.32
1,770.22
|
|
| Submitter | Shuang Jiang (271339616@qq.com) |
|---|
| Organization | Shanghai Academy of Agricultural Sciences |
|---|
| Date submitted | 2022-10-26 |
|---|
