| Accession | CRX534730 |
| Organism | Olea europaea subsp. europaea |
| Title | Manzanilla_3h_2 |
| BioProject | PRJCA012898 |
| BioSample | SAMC974927 |
| Platform | Illumina NovaSeq 5000 |
| Library |
| Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
The mRNA was enriched by binding to the ployA tail of the mRNA by A-T complementary pairing using magnetic beads with Oligo (dT). Then fragmentation buffer was added to break the mRNA into short fragments. The mRNA was used as a template to synthesize the first strand cDNA with random hexamers, and then buffer, dNTPs and DNA polymerase I were added to synthesize the second strand cDNA. Double-stranded cDNA was subsequently purified using AMPure XP beads. The purified double-stranded cDNA was then subjected to end repair, A-tail addition and sequencing adapter ligation, followed by fragment size selection with AMPure XP beads, and finally PCR enrichment to obtain the final cDNA library. |
RNA-Seq |
TRANSCRIPTOMIC |
PolyA |
PAIRED
|
|
| Processing |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
|
| Release date | 2024-11-01 |
|---|
| Run |
| Run accession |
Run data file information |
| File name | File size (MB) |
|---|
| CRR599859 |
CRR599859_f1.fq.gz
CRR599859_r2.fq.gz
|
1,322.13
1,385.43
|
|
| Submitter | Tianyi Wang (wangty02@163.com) |
|---|
| Organization | Chinese Academy of Forestry |
|---|
| Date submitted | 2022-11-04 |
|---|
