| 实验编号 | CRX543185 |
| 物种名称 | Mus musculus |
| 标题 | E3_Tr3 |
| 项目编号 | PRJCA013079 |
| 样本编号 | SAMC987527 |
| 测序平台 | Illumina HiSeq X Ten |
| 建库信息 |
| 文库名称 |
文库构建方法 |
建库策略 |
样品来源 |
实验选择 |
文库布局 |
|
1 μg total RNA was used for following library preparation. The poly(A) mRNA isolation was performed using Oligo(dT) beads. The mRNA fragmentation was performed using divalent cations and high temperature. Priming was performed using Random Primers. First strand cDNA and the second-strand cDNA were synthesized. The purified double-stranded cDNA was then treated to repair both ends and add a dA-tailing in one reaction, followed by a T-A ligation to add adaptors to both ends. Size selection of
Adaptor-ligated DNA was then performed using DNA Clean Beads. Each sample was then amplified by
PCR using P5 and P7 primers and the PCR products were validated. |
RNA-Seq |
TRANSCRIPTOMIC |
PCR |
PAIRED
|
|
| 处理信息 |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
|
| 发布日期 | 2022-11-09 |
|---|
| 测序反应 |
| Run编号 |
Run序列文件信息 |
| File name | File size (MB) |
|---|
| CRR608409 |
CRR608409_f1.fastq.gz
CRR608409_r2.fastq.gz
|
1,744.09
1,841.23
|
|
| 提交者 | ZOEY ZHU (1239957644@qq.com) |
|---|
| 所属单位 | The Affiliated Hospital of Nanjing University Medical School |
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| 提交日期 | 2022-11-09 |
|---|
