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Experiment information
Accession CRX543238
Organism Mus musculus
Title HHcy_rep4
BioProject PRJCA013099
BioSample SAMC988173
Platform Illumina HiSeq 2500
Library
Library name Construction protocol Strategy Source Selection Layout
Total RNA was extracted using Trizol reagent kit (Invitrogen, Carlsbad, CA,USA) protocol. After total RNA was extracted, eukaryotic mRNA was enriched, fragmented and reverse transcripted into cDNA. Then the cDNA fragments were purified with QiaQuick PCR extraction kit(Qiagen, Venlo, The Netherlands), end repaired, poly(A) added, and ligated to Illumina sequencing adapters.The hyperhomocysteinemia mice were fed with 19.56g/Kg methionine diet and 1.8g/L DL-homocysteine water. RNA-Seq TRANSCRIPTOMIC PCR PAIRED
Processing Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Release date2024-11-09
Run
Run accession Run data file information
File nameFile size (MB)
CRR608472 CRR608472_f1.fq.gz
CRR608472_r2.fq.gz
2,041.48
2,071.82
SubmitterJing Nie (niejing@smu.edu.cn)
OrganizationNanfang Hospital, Southern Medical University
Date submitted2022-11-09
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