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Experiment information
Accession CRX552214
Organism Mus musculus
Title Mouse_embryos_E6.5_vivo
BioProject PRJCA013348
BioSample SAMC998452
Platform Illumina HiSeq 4000
Library
Library name Construction protocol Strategy Source Selection Layout
scRNA-seq libraries were constructed based on the modified single-cell tagged reverse transcription sequencing protocol. The single cell mRNAs in lysates were reverse-transcribed into the cDNAs with a primer containing a barcode and unique molecular identifiers. Subsequently, cDNAs with cell-specific barcodes were pooled together after amplification The cDNA products purified were indexed at the 3' ends of the cDNAs with biotin-amplification, followed by fragments of cDNAs using a Covaris system. Fragments with biotin tag at the 3' ends were enriched using Dynabeads MyOne Streptavidin C1 beads . Ultimately, the libraries were constructed using Kapa Hyper Prep Kits and sequenced on an Illumina HiSeq 4000 platform. RNA-Seq TRANSCRIPTOMIC SINGLE CELL PolyA PAIRED
Processing Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Release date2022-11-24
Run
Run accession Run data file information
File nameFile size (MB)
CRR617212 CRR617212_f1.fastq.gz
CRR617212_r2.fastq.gz 		8,622.33
8,561.61
CRR617213 CRR617213_f1.fastq.gz
CRR617213_r2.fastq.gz 		8,476.41
9,047.26
CRR617214 CRR617214_f1.fastq.gz
CRR617214_r2.fastq.gz 		9,067.91
8,804.89
SubmitterQingyuan Zhu (zhuqingyuan@mail.tsinghua.edu.cn)
OrganizationTsinghua University
Date submitted2022-11-19
Related experiments
Experiments(1)