| 实验编号 | CRX552214 |
| 物种名称 | Mus musculus |
| 标题 | Mouse_embryos_E6.5_vivo |
| 项目编号 | PRJCA013348 |
| 样本编号 | SAMC998452 |
| 测序平台 | Illumina HiSeq 4000 |
| 建库信息 |
| 文库名称 |
文库构建方法 |
建库策略 |
样品来源 |
实验选择 |
文库布局 |
|
scRNA-seq libraries were constructed based on the modified single-cell tagged reverse transcription sequencing protocol. The single cell mRNAs in lysates were reverse-transcribed into the cDNAs with a primer containing a barcode and unique molecular identifiers. Subsequently, cDNAs with cell-specific barcodes were pooled together after amplification The cDNA products purified were indexed at the 3' ends of the cDNAs with biotin-amplification, followed by fragments of cDNAs using a Covaris system. Fragments with biotin tag at the 3' ends were enriched using Dynabeads MyOne Streptavidin C1 beads . Ultimately, the libraries were constructed using Kapa Hyper Prep Kits and sequenced on an Illumina HiSeq 4000 platform. |
RNA-Seq |
TRANSCRIPTOMIC SINGLE CELL |
PolyA |
PAIRED
|
|
| 处理信息 |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
|
| 发布日期 | 2022-11-24 |
|---|
| 测序反应 |
| Run编号 |
Run序列文件信息 |
| File name | File size (MB) |
|---|
| CRR617212 |
CRR617212_f1.fastq.gz
CRR617212_r2.fastq.gz
|
8,622.33
8,561.61
|
| CRR617213 |
CRR617213_f1.fastq.gz
CRR617213_r2.fastq.gz
|
8,476.41
9,047.26
|
| CRR617214 |
CRR617214_f1.fastq.gz
CRR617214_r2.fastq.gz
|
9,067.91
8,804.89
|
|
| 提交者 | Qingyuan Zhu (zhuqingyuan@mail.tsinghua.edu.cn) |
|---|
| 所属单位 | Tsinghua University |
|---|
| 提交日期 | 2022-11-19 |
|---|
