| 实验编号 | CRX552215 |
| 物种名称 | Mus musculus |
| 标题 | Mouse_embryos_E6.5_vitro |
| 项目编号 | PRJCA013348 |
| 样本编号 | SAMC998453 |
| 测序平台 | Illumina HiSeq 4000 |
| 建库信息 |
| 文库名称 |
文库构建方法 |
建库策略 |
样品来源 |
实验选择 |
文库布局 |
|
scRNA-seq libraries were constructed based on the modified single-cell tagged reverse transcription sequencing protocol. The single cell mRNAs in lysates were reverse-transcribed into the cDNAs with a primer containing a barcode and unique molecular identifiers. Subsequently, cDNAs with cell-specific barcodes were pooled together after amplification The cDNA products purified were indexed at the 3' ends of the cDNAs with biotin-amplification, followed by fragments of cDNAs using a Covaris system. Fragments with biotin tag at the 3' ends were enriched using Dynabeads MyOne Streptavidin C1 beads . Ultimately, the libraries were constructed using Kapa Hyper Prep Kits and sequenced on an Illumina HiSeq 4000 platform. |
RNA-Seq |
TRANSCRIPTOMIC SINGLE CELL |
PolyA |
PAIRED
|
|
| 处理信息 |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
|
| 发布日期 | 2022-11-24 |
|---|
| 测序反应 |
| Run编号 |
Run序列文件信息 |
| File name | File size (MB) |
|---|
| CRR617215 |
CRR617215_f1.fastq.gz
CRR617215_r2.fastq.gz
|
8,175.52
7,983.36
|
| CRR617216 |
CRR617216_f1.fastq.gz
CRR617216_r2.fastq.gz
|
8,294.52
8,510.19
|
| CRR617217 |
CRR617217_f1.fastq.gz
CRR617217_r2.fastq.gz
|
9,727.55
8,938.47
|
| CRR617218 |
CRR617218_f1.fastq.gz
CRR617218_r2.fastq.gz
|
7,863.72
7,416.62
|
|
| 提交者 | Qingyuan Zhu (zhuqingyuan@mail.tsinghua.edu.cn) |
|---|
| 所属单位 | Tsinghua University |
|---|
| 提交日期 | 2022-11-19 |
|---|
