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Experiment information
Accession CRX552215
Organism Mus musculus
Title Mouse_embryos_E6.5_vitro
BioProject PRJCA013348
BioSample SAMC998453
Platform Illumina HiSeq 4000
Library
Library name Construction protocol Strategy Source Selection Layout
scRNA-seq libraries were constructed based on the modified single-cell tagged reverse transcription sequencing protocol. The single cell mRNAs in lysates were reverse-transcribed into the cDNAs with a primer containing a barcode and unique molecular identifiers. Subsequently, cDNAs with cell-specific barcodes were pooled together after amplification The cDNA products purified were indexed at the 3' ends of the cDNAs with biotin-amplification, followed by fragments of cDNAs using a Covaris system. Fragments with biotin tag at the 3' ends were enriched using Dynabeads MyOne Streptavidin C1 beads . Ultimately, the libraries were constructed using Kapa Hyper Prep Kits and sequenced on an Illumina HiSeq 4000 platform. RNA-Seq TRANSCRIPTOMIC SINGLE CELL PolyA PAIRED
Processing Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Release date2022-11-24
Run
Run accession Run data file information
File nameFile size (MB)
CRR617215 CRR617215_f1.fastq.gz
CRR617215_r2.fastq.gz 		8,175.52
7,983.36
CRR617216 CRR617216_f1.fastq.gz
CRR617216_r2.fastq.gz 		8,294.52
8,510.19
CRR617217 CRR617217_f1.fastq.gz
CRR617217_r2.fastq.gz 		9,727.55
8,938.47
CRR617218 CRR617218_f1.fastq.gz
CRR617218_r2.fastq.gz 		7,863.72
7,416.62
SubmitterQingyuan Zhu (zhuqingyuan@mail.tsinghua.edu.cn)
OrganizationTsinghua University
Date submitted2022-11-19
Related experiments
Experiments(1)