Experiment information
Accession CRX553022
Organism Mus
Title A24_1
BioProject PRJCA013425
BioSample SAMC1004491
Platform Illumina NovaSeq 6000
Library
Library name Construction protocol Strategy Source Selection Layout
Total RNA was extracted from the testicular tissues of animals in the alcohol-FMT and control-FMT groups, with three replicates in each group. RNA integrity was assessed using an RNA Nano 6000 Assay Kit and a Bioanalyzer 2100 system (Agilent Technologies, Santa Clara, CA, USA). The cDNA libraries were prepared using an Illumina TruSeq RNA Sample Prep Kit. The average size of the library cDNAs was 150 bp (excluding adapters). The integrity and quality of cDNA libraries were assessed using an Agilent 2100 Bioanalyzer and an ABI StepOne Plus real-time PCR system. RNA-Seq TRANSCRIPTOMIC PCR PAIRED
Processing Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Release date2022-11-24
Run
Run accession Run data file information
File nameFile size (MB)
CRR618085 CRR618085_f1.fq.gz
CRR618085_r2.fq.gz
1,459.55
1,486.16
Submitterhui li (lihui123@tmmu.edu.cn)
OrganizationXinqiao Hospital of Army Medical University
Date submitted2022-11-24
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