| Accession | CRX572114 |
| Organism | Mus musculus |
| Title | Snhg3-LKI-HFD1 |
| BioProject | PRJCA013840 |
| BioSample | SAMC1041308 |
| Platform | BGISEQ-500 |
| Library |
| Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
Fresh tissue sample is flash frozen by liquid nitrogen and then ground completely. The transposition reactions are initiated by adding transposase.The PCR reaction system is configured to initiate PCR amplification of the transposition products.The corresponding library quality control protocol will be selected depending upon product requirements.Single-stranded PCR products are produced via denaturation. The reaction system and program for circularization are subsequently configured and set up. Single-stranded cyclized products are produced, while uncyclized linear DNA molecules are digested.Single-stranded circle DNA molecules are replicated via rolling cycle amplification, and a DNA nanoball (DNB) which contain multiple copies of DNA is generated. Sufficient quality DNBs are then loaded into patterned nanoarrays using high-intensity DNA nanochip technique and sequenced through combinatorial Probe-Anchor Synthesis (cPAS). |
ATAC-seq |
GENOMIC |
PCR |
SINGLE
|
|
| Processing |
Planned read length (bp): 100
|
| Release date | 2024-10-21 |
|---|
| Run |
| Run accession |
Run data file information |
| File name | File size (MB) |
|---|
| CRR640369 |
CRR640369.bam
|
43,847.33
|
|
| Submitter | Xiaojun liu Liu (xiaojunliu@ibms.pumc.edu.cn) |
|---|
| Organization | Institute of Basic Medical Sciences Chinese Academy of Medical Sciences & School of Basic Medicine Peking Union Medical College, |
|---|
| Date submitted | 2023-01-10 |
|---|
