Accession | CRX572675 |
Organism | Mus musculus |
Accession in Other Database |
: |
Title | OE1 |
BioProject | PRJCA014366 |
BioSample | SAMC1041776 |
Platform | Illumina HiSeq X Ten |
Library |
Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
mRNA was purified from total RNA using poly-T oligo-attached magnetic beads. Fragmentation was carried out using divalent cations under elevated temperature in NEBNext First Strand Synthesis Reaction Buffer (5X). First strand cDNA was synthesized using random hexamer primer and RNase H. Second strand cDNA synthesis was subsequently performed using buffer, dNTPs, DNA polymerase I and RNase H. The library fragments were purified with QiaQuick PCR kits and elution with EB buffer, then terminal repair, A-tailing and adapter added were implemented. The aimed products were retrieved and PCR was performed, then the library was completed. |
RNA-Seq |
TRANSCRIPTOMIC |
PCR |
PAIRED
|
|
Processing |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
|
Release date | 2025-01-11 |
Run |
Run accession |
Run data file information |
File name | File size (MB) |
CRR640932 |
CRR640932_f1.fq.gz
CRR640932_r2.fq.gz
|
1,427.85
1,471.33
|
|
Submitter | LIU CHANGMEI (liuchm@ioz.ac.cn) |
Organization | Institute of Zoology, Chinese Academy of Sciences |
Date submitted | 2023-01-11 |