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Experiment information
Accession CRX572679
Organism Mus musculus
Title WT2
BioProject PRJCA014366
BioSample SAMC1041780
Platform Illumina HiSeq X Ten
Library
Library name Construction protocol Strategy Source Selection Layout
mRNA was purified from total RNA using poly-T oligo-attached magnetic beads. Fragmentation was carried out using divalent cations under elevated temperature in NEBNext First Strand Synthesis Reaction Buffer (5X). First strand cDNA was synthesized using random hexamer primer and RNase H. Second strand cDNA synthesis was subsequently performed using buffer, dNTPs, DNA polymerase I and RNase H. The library fragments were purified with QiaQuick PCR kits and elution with EB buffer, then terminal repair, A-tailing and adapter added were implemented. The aimed products were retrieved and PCR was performed, then the library was completed. RNA-Seq TRANSCRIPTOMIC PCR PAIRED
Processing Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Release date2025-01-11
Run
Run accession Run data file information
File nameFile size (MB)
CRR640936 CRR640936_f1.fq.gz
CRR640936_r2.fq.gz
1,468.94
1,491.99
SubmitterLIU CHANGMEI (liuchm@ioz.ac.cn)
OrganizationInstitute of Zoology, Chinese Academy of Sciences
Date submitted2023-01-11
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