| 实验编号 | CRX583054 |
| 物种名称 | Mus musculus |
| 标题 | LARP4_RIP-seq |
| 项目编号 | PRJCA009531 |
| 样本编号 | SAMC1065638 |
| 测序平台 | Illumina NovaSeq 5000 |
| 建库信息 |
| 文库名称 |
文库构建方法 |
建库策略 |
样品来源 |
实验选择 |
文库布局 |
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RNA immunoprecipitation (RIP) was performed with an EZ-Magna RIP Kit.Briefly, First, 2 × 107 naive CD4+ T cells were lysed by RIP lysis buffer and frozen overnight at -80°C. Then, immunoprecipitation (IP) was performed using anti-LARP4 antibodies, and the antibody-enriched RNAs were extracted and purified after digestion of DNA and proteins. These enriched and purified RNAs and input RNA were subject to analysis by high-throughput sequencing (DIATRE Technology Ltd). |
RIP-Seq |
TRANSCRIPTOMIC |
RANDOM PCR |
PAIRED
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|
| 处理信息 |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
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| 发布日期 | 2025-06-17 |
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| 测序反应 |
| Run编号 |
Run序列文件信息 |
| File name | File size (MB) |
|---|
| CRR653119 |
CRR653119_f1.fastq.gz
CRR653119_r2.fastq.gz
|
1,133.43
1,193.62
|
| CRR653120 |
CRR653120_f1.fastq.gz
CRR653120_r2.fastq.gz
|
1,457.32
1,555.08
|
|
| 提交者 | Yi Tian (tianyi19831015@hotmail.com) |
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| 所属单位 | Third Military Medical University(Army Medical University) |
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| 提交日期 | 2023-02-11 |
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