Accession | CRX637420 |
Organism | Bacteria |
Title | pe-1-1 |
BioProject | PRJCA015536 |
BioSample | SAMC1153856 |
Platform | Illumina HiSeq 2500 |
Library |
Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
The DNA of sludge samples were extracted. Primers 515F (5’-GTGCCAGCMGCCGCGGTAA-3’) and 818R (5’-GGACTACHVGGGTWTCTAAT-3’) were used for 16S rRNA gene V4 region amplification. The PCR products with different barcodes were purified, quantified, and pooled tighter to construct the DNA library, and high throughput sequencing was performed on Illumina sequencing platform. |
AMPLICON |
OTHER |
PCR |
PAIRED
|
|
Processing |
Planned read length (bp) for mate 1: 250
Planned read length (bp) for mate 2: 250
|
Release date | 2023-03-16 |
Run |
Run accession |
Run data file information |
File name | File size (MB) |
CRR711335 |
CRR711335_f1.fq.gz
CRR711335_r2.fq.gz
|
9.23
8.47
|
|
Submitter | Qiao Ma (xiaoma0556@qq.com) |
Organization | Dalian Maritime University |
Date submitted | 2023-03-16 |