| Accession | CRX638162 |
| Organism | human gut metagenome |
| Title | ABS-3 |
| BioProject | PRJCA008334 |
| BioSample | SAMC1154979 |
| Platform | Illumina HiSeq X Ten |
| Library |
| Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
DNA for each sample was extracted with TruSeq Nano DNA LT Sample Prepararion Kit, sheared to approximately 300-500 bp using a Covaris S220 instrument. Then the libraries were constructed through end-repair, A-tailing, adapter ligation. Then it was sequenced on Illumina platform in PE150 mode. The Metagenome sequencing and analysis were conducted by OE biotech Co., Ltd. The raw data was in FASTQ format. Reads were trimmed and filtered using Trimmomatic. Metagenome assembly was performed using MEGAHIT after getting valid reads. ORF prediction of assembled scaffolds using prodigal was performed and translated into amino acid sequences. clean reads of each sample were aligned against the non-redundant gene set use bowtie4. and the abundant information of the gene in the corresponding sample was counted. |
WGS |
METAGENOMIC |
unspecified |
PAIRED
|
|
| Processing |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
|
| Release date | 2023-03-22 |
|---|
| Run |
| Run accession |
Run data file information |
| File name | File size (MB) |
|---|
| CRR712081 |
CRR712081_f1.fastq.gz
CRR712081_r2.fastq.gz
|
4,795.19
4,779.08
|
|
| Submitter | Jing Yuan (yuanjing6216@163.com) |
|---|
| Organization | Capital Institute of Pediatrics |
|---|
| Date submitted | 2023-03-17 |
|---|
