| 实验编号 | CRX641426 |
| 物种名称 | Bos taurus |
| 标题 | MK_SM_3 |
| 项目编号 | PRJCA015714 |
| 样本编号 | SAMC1163459 |
| 测序平台 | Illumina NovaSeq 6000 |
| 建库信息 |
| 文库名称 |
文库构建方法 |
建库策略 |
样品来源 |
实验选择 |
文库布局 |
|
After the total RNA was qualified, the eukaryotic mRNA was enriched with magnetic beads attached with Oligo(dT).AMPureXP beads purify double strand beads. T4 DNA polymerase and Klenow DNA polymerase activities are used to repair the adhesive ends of DNA into flat ends. Base A and joint are added to the 5 'ends of DNA.Finally, PCR amplification was performed to obtain the final sequencing library. |
RNA-Seq |
TRANSCRIPTOMIC |
PCR |
PAIRED
|
|
| 处理信息 |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
|
| 发布日期 | 2023-05-01 |
| 测序反应 |
| Run编号 |
Run序列文件信息 |
| File name | File size (MB) |
| CRR716438 |
CRR716438_f1.fq.gz
CRR716438_r2.fq.gz
|
1,356.79
1,477.45
|
|
| 提交者 | mingjuan gu (gmj0119@yeah.net) |
| 所属单位 | Inner Mongolia University |
| 提交日期 | 2023-03-23 |