logo
实验基本信息
实验编号 CRX641433
物种名称 Bos taurus
标题 WT_SM_5
项目编号 PRJCA015714
样本编号 SAMC1163466
测序平台 Illumina NovaSeq 6000
建库信息
文库名称 文库构建方法 建库策略 样品来源 实验选择 文库布局
After the total RNA was qualified, the eukaryotic mRNA was enriched with magnetic beads attached with Oligo(dT).AMPureXP beads purify double strand beads. T4 DNA polymerase and Klenow DNA polymerase activities are used to repair the adhesive ends of DNA into flat ends. Base A and joint are added to the 12 'ends of DNA.Finally, PCR amplification was performed to obtain the final sequencing library. RNA-Seq TRANSCRIPTOMIC PCR PAIRED
处理信息 Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
发布日期2023-05-01
测序反应
Run编号 Run序列文件信息
File nameFile size (MB)
CRR716445 CRR716445_f1.fq.gz
CRR716445_r2.fq.gz
1,174.49
1,276.02
提交者mingjuan gu (gmj0119@yeah.net)
所属单位Inner Mongolia University
提交日期2023-03-23