| Accession | CRX641868 |
| Organism | Brassica juncea |
| Title | RNA-Seq of cotyledon |
| BioProject | PRJCA015688 |
| BioSample | SAMC1164181 |
| Platform | Illumina NovaSeq 6000 |
| Library |
| Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
RNA were extracted with TRlzol Reagent (Life technologies, California, USA). Concentration and integrity of RNA sample were examined by NanoDrop and Agilent 2100 (Agilent Technologies). Then, the enriched mRNA were fragmented into approximately 200nt RNA inserts, which were used to synthesize the first-strand cDNA and the second cDNA. Suitable fragments were isolated by Agencourt AMPure XP beads (Beckman), and enriched by PCR amplification. RNA-sequencing librarys construction were made by using the NEBNext Ultra RNA Library Prep Kit for Illumina (NEB) following the manufacturer’s recommendations. |
RNA-Seq |
TRANSCRIPTOMIC |
PCR |
PAIRED
|
|
| Processing |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
|
| Release date | 2025-05-10 |
|---|
| Run |
| Run accession |
Run data file information |
| File name | File size (MB) |
|---|
| CRR716892 |
CRR716892_f1.fq.gz
CRR716892_r2.fq.gz
|
1,165.46
1,242.8
|
|
| Submitter | Shuangping Heng (shuangpingheng@126.com) |
|---|
| Organization | Xinyang normal university |
|---|
| Date submitted | 2023-03-23 |
|---|
