| 实验编号 | CRX641872 |
| 物种名称 | Brassica juncea |
| 标题 | RNA-Seq of sillique |
| 项目编号 | PRJCA015688 |
| 样本编号 | SAMC1164185 |
| 测序平台 | Illumina NovaSeq 6000 |
| 建库信息 |
| 文库名称 |
文库构建方法 |
建库策略 |
样品来源 |
实验选择 |
文库布局 |
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RNA were extracted with TRlzol Reagent (Life technologies, California, USA). Concentration and integrity of RNA sample were examined by NanoDrop and Agilent 2100 (Agilent Technologies). Then, the enriched mRNA were fragmented into approximately 200nt RNA inserts, which were used to synthesize the first-strand cDNA and the second cDNA. Suitable fragments were isolated by Agencourt AMPure XP beads (Beckman), and enriched by PCR amplification. RNA-sequencing librarys construction were made by using the NEBNext Ultra RNA Library Prep Kit for Illumina (NEB) following the manufacturer’s recommendations. |
RNA-Seq |
TRANSCRIPTOMIC |
PCR |
PAIRED
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|
| 处理信息 |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
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| 发布日期 | 2025-05-10 |
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| 测序反应 |
| Run编号 |
Run序列文件信息 |
| File name | File size (MB) |
|---|
| CRR716896 |
CRR716896_f1.fq.gz
CRR716896_r2.fq.gz
|
1,306.91
1,370.29
|
|
| 提交者 | Shuangping Heng (shuangpingheng@126.com) |
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| 所属单位 | Xinyang normal university |
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| 提交日期 | 2023-03-23 |
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