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Fresh young leaf tissue was collected and immediately frozen in liquid nitrogen, and high-quality DNA was isolated from fresh young leaves using the CTAB method and assessed with the NanoDrop One spectrophotometer (NanoDrop Technologies, Wilmington, DE)). The DNA quantity was measured with a Qubit 3.0 Fluorometer (Life Technologies, Carlsbad, CA, USA). For ONT ultra-long sequencing, the standard library was prepared using the SQK-ULK001 kit, following the standard protocol. The purified library was sequenced using a PromethION sequencer (Oxford Nanopore Technologies, Oxford, UK). |
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