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文库构建方法 |
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Qualified genomic DNA was randomly interrupted with enzymes, then terminal repair was performed, an A-tail was added, and Illumina sequencing splices were added using the NEBNextMLtraDNA Library Prep Kit (NEB,USA). 300-400bp DNA fragments were amplified and enriched by PCR. Finally, the PCR products were purified by AMPure XP system (Beckman Coulter.Brea.CA.USA). Sequencing libraries were examined using Agilent 210g biological analyzer (Agilent, Santa Clara,CA) and quantified using real-time PCR. The sequencing was performed on the Novaseq 6000 sequencer, using the PE 196 sequencing strategy. |
WGS |
GENOMIC |
PolyA |
PAIRED
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