| Accession | CRX722150 |
| Organism | Juglans regia |
| Title | Juglanshindsiinortherncaliforniawalnut_AD237-01-T05 |
| BioProject | PRJCA017598 |
| BioSample | SAMC1317357 |
| Platform | Illumina NovaSeq 5000 |
| Library |
| Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
| AD237-01-T05 |
The initial RNA was total RNA, total RNA > = 1ug. The library building kit used in library building is Illumina NEBNext ® UltraTM RNA Library Prep Kit. The mRNA with polyA tail was enriched by Oligo ( dT ) beads, and then the mRNA was randomly interrupted by divalent cations in NEB Fragmentation Buffer. The first strand of cDNA was synthesized in M-MuLV reverse transcriptase system with random oligonucleotide primers and fragmentized mRNA as template. RNaseH degrades RNA strand and synthesizes dNTPs in DNA polymerase I system Make the second strand of cDNA. Purified double-stranded cDNA was repaired by terminal repair Add A tail and connect the sequencing joint, and use AMPure XP beads to screen about 250 ~ 304 bp cDNA, amplified by PCR and purified by AMPure XP beads, Finally, the library was obtained. |
OTHER |
TRANSCRIPTOMIC |
size fractionation |
PAIRED
|
|
| Processing |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
|
| Release date | 2023-06-20 |
|---|
| Run |
| Run accession |
Run data file information |
| File name | File size (MB) |
|---|
| CRR803942 |
CRR803942_f1.fq.gz
CRR803942_r2.fq.gz
|
1,644.44
1,672.72
|
|
| Submitter | Liu Kai (walnutlk@163.com) |
|---|
| Organization | Hebei Agricultural University |
|---|
| Date submitted | 2023-06-16 |
|---|
