Accession | CRX722161 |
Organism | Juglans regia |
Title | Juglanshindsiinortherncaliforniawalnut_AD237-01-T16 |
BioProject | PRJCA017598 |
BioSample | SAMC1317368 |
Platform | Illumina NovaSeq 5000 |
Library |
Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
AD237-01-T16 |
The initial RNA was total RNA, total RNA > = 1ug. The library building kit used in library building is Illumina NEBNext ® UltraTM RNA Library Prep Kit. The mRNA with polyA tail was enriched by Oligo ( dT ) beads, and then the mRNA was randomly interrupted by divalent cations in NEB Fragmentation Buffer. The first strand of cDNA was synthesized in M-MuLV reverse transcriptase system with random oligonucleotide primers and fragmentized mRNA as template. RNaseH degrades RNA strand and synthesizes dNTPs in DNA polymerase I system Make the second strand of cDNA. Purified double-stranded cDNA was repaired by terminal repair Add A tail and connect the sequencing joint, and use AMPure XP beads to screen about 250 ~ 315 bp cDNA, amplified by PCR and purified by AMPure XP beads, Finally, the library was obtained. |
OTHER |
TRANSCRIPTOMIC |
size fractionation |
PAIRED
|
|
Processing |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
|
Release date | 2023-06-20 |
Run |
Run accession |
Run data file information |
File name | File size (MB) |
CRR803953 |
CRR803953_f1.fq.gz
CRR803953_r2.fq.gz
|
1,583.12
1,619.42
|
|
Submitter | Liu Kai (walnutlk@163.com) |
Organization | Hebei Agricultural University |
Date submitted | 2023-06-16 |