| 实验编号 | CRX1007838 |
| 物种名称 | Mus musculus |
| 标题 | CUT&RUN data for ELL3 in 2,5-HD-treated mESC (replicate 1) |
| 项目编号 | PRJCA018079 |
| 样本编号 | SAMC3487596 |
| 测序平台 | Illumina NovaSeq 6000 |
| 建库信息 |
| 文库名称 |
文库构建方法 |
建库策略 |
样品来源 |
实验选择 |
文库布局 |
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The CUT&RUN assay was conducted on 0.2 million cells per sample, utilizing the Hyperactive pG-MNase CUT&RUN assay kit (Vazyme, HD102) with slightly modifications to the manufacturer’s protocol. The libraries were prepared using NEBNext Ultra II DNA library prep kit (NEB, E7645) with modified amplification condition as 98 °C for 30 sec, 15 cycles of 98 °C for 10 sec and 65°C for 17 sec, final extension at 65 °C for 2 min and hold at 4°C. |
OTHER |
GENOMIC |
other |
PAIRED
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|
| 处理信息 |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
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| 发布日期 | 2024-06-24 |
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| 测序反应 |
| Run编号 |
Run序列文件信息 |
| File name | File size (MB) |
|---|
| CRR1095322 |
CRR1095322_f1.fastq.gz
CRR1095322_r2.fastq.gz
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2,814.06
2,931.89
|
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| 提交者 | Yong Zhang (yzhang@tongji.edu.cn) |
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| 所属单位 | Tongji University |
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| 提交日期 | 2024-04-02 |
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