| Library |
| Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
Oligo(dT) beads were used to enrich mRNA with polyA structure in total RNA, and cDNA was synthesized using 6-base random primer and reverse transcriptase as template. cDNA was enriched by PCR amplification after library construction, and the library was selected according to the size of the fragments, which was 450 bp. Then the library was analyzed by Agilent 2100 Bioanalyzer. Bioanalyzer for quality control, and then the total concentration of the library and the effective concentration of the library were detected. The mixed libraries are uniformly diluted to 3nM and denatured by base denaturation to form single-stranded libraries. After RNA extraction, purification and library construction, the samples were subjected to double-end sequencing based on the Illumina HiSeq sequencing platform. |
RNA-Seq |
TRANSCRIPTOMIC |
RANDOM PCR |
PAIRED
|
|
