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实验基本信息
实验编号 CRX757192
物种名称 Dendostrea crenulifera
标题 Transcriptom of Dendostrea crenulifera
项目编号 PRJCA018809
样本编号 SAMC2975823
测序平台 Illumina HiSeq 4000
建库信息
文库名称 文库构建方法 建库策略 样品来源 实验选择 文库布局
1803686 3 oysters were sampled from each sample. Whole body from each oyster was flash-frozen in liquid nitrogen and used for transcriptome sequencing. Total RNA was extracted from the whole body using TRIzol reagent (Invitrogen,USA) following the manufacturer’s protocol. The quantity and quality of the extracted total RNA were assessed with a A total of 3 mg of RNA per group was used for library preparation. Sequencing libraries were generated using an NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (NEB, USA) following the manufacturer’s recommendations, and unique index codes were utilised to distinguish the samples. RNA-Seq TRANSCRIPTOMIC PolyA PAIRED
处理信息 Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
发布日期2023-08-09
测序反应
Run编号 Run序列文件信息
File nameFile size (MB)
CRR840612 CRR840612_f1.fastq.gz
CRR840612_r2.fastq.gz
2,426.87
2,715.55
提交者CUI LI (licui19877322@163.com)
所属单位Institute of Oceanology, Chinese Academy of Sciences
提交日期2023-08-04