| 建库信息 |
| 文库名称 |
文库构建方法 |
建库策略 |
样品来源 |
实验选择 |
文库布局 |
|
High-quality genomic DNA was isolated from fresh leaf tissue using the Qiagen DNeasy Plant Mini Kit. MGI libraries were constructed by using the MGIEasy Universal DNA Library Prep Set (BGI, China). In brief, 1-1.5 μg of genomic DNA was randomly fragmented with a Covaris instrument. Then, fragments with sizes between 200 and 400 bp were selected using an Agencourt AMPure XP-Medium Kit, followed by end repair, 3' adenylation and adapter ligation. After PCR enrichment, the PCR products were recovered using the AxyPrep Mag PCR clean-up kit. The double-stranded PCR products were heat denatured and circularized using the splint oligo sequence. Single-stranded circular DNA (ssCir DNA) was formatted as the final library and qualified according to QC procedures. The qualified libraries were sequenced on the MGISEQ2000 platform at BGI (Wuhan, China). |
WGS |
GENOMIC |
RANDOM |
PAIRED
|
|
| 测序反应 |
| Run编号 |
Run序列文件信息 |
| File name | File size (MB) |
|---|
| CRR843458 |
CRR843458_f1.fq.gz
CRR843458_r2.fq.gz
|
9,068.07
9,676.12
|
| CRR843459 |
CRR843459_f1.fq.gz
CRR843459_r2.fq.gz
|
8,978.43
9,687.29
|
| CRR843460 |
CRR843460_f1.fq.gz
CRR843460_r2.fq.gz
|
9,068.7
9,675.18
|
| CRR843461 |
CRR843461_f1.fq.gz
CRR843461_r2.fq.gz
|
8,978.45
9,687.2
|
| CRR843462 |
CRR843462_f1.fq.gz
CRR843462_r2.fq.gz
|
9,070.48
9,626.83
|
| CRR843463 |
CRR843463_f1.fq.gz
CRR843463_r2.fq.gz
|
8,937.92
9,601.55
|
| CRR843464 |
CRR843464_f1.fq.gz
CRR843464_r2.fq.gz
|
9,070.62
9,627.2
|
| CRR843465 |
CRR843465_f1.fq.gz
CRR843465_r2.fq.gz
|
8,938.28
9,602.27
|
| CRR843466 |
CRR843466_f1.fq.gz
CRR843466_r2.fq.gz
|
9,087.37
9,618.68
|
| CRR843467 |
CRR843467_f1.fq.gz
CRR843467_r2.fq.gz
|
8,936.69
9,584.95
|
| CRR843468 |
CRR843468_f1.fq.gz
CRR843468_r2.fq.gz
|
9,087.68
9,619
|
| CRR843469 |
CRR843469_f1.fq.gz
CRR843469_r2.fq.gz
|
8,936.93
9,585.12
|
| CRR843470 |
CRR843470_f1.fq.gz
CRR843470_r2.fq.gz
|
9,052.8
9,636.06
|
| CRR843471 |
CRR843471_f1.fq.gz
CRR843471_r2.fq.gz
|
9,033.04
9,697.03
|
| CRR843472 |
CRR843472_f1.fq.gz
CRR843472_r2.fq.gz
|
9,052.61
9,635.6
|
| CRR843473 |
CRR843473_f1.fq.gz
CRR843473_r2.fq.gz
|
9,032.99
9,696.5
|
| CRR843474 |
CRR843474_f1.fq.gz
CRR843474_r2.fq.gz
|
2,430.66
2,586.19
|
| CRR843475 |
CRR843475_f1.fq.gz
CRR843475_r2.fq.gz
|
2,461.04
2,667.31
|
| CRR843476 |
CRR843476_f1.fq.gz
CRR843476_r2.fq.gz
|
2,392.44
2,545.5
|
| CRR843477 |
CRR843477_f1.fq.gz
CRR843477_r2.fq.gz
|
2,518.34
2,729.53
|
|
