Accession | CRX772458 |
Organism | Arachis monticola |
Title | Long-read sequencing of Arachis monticola |
BioProject | PRJCA019219 |
BioSample | SAMC3013710 |
Platform | OXFORD_NANOPORE PromethION |
Library |
Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
A total amount of 2 µg DNA per sample was used as input material for the ONT library preparations. After the sample was qualified, size-select of long DNA fragments were performed using the BluePippin system (Sage Science, USA). Next, the ends of DNA fragments were repaired and A-ligation reaction were conducted with NEBNext Ultra II End Repair/dA-tailing Kit (Cat# E7546). The adapter in the LSK109 kit was used for further ligation reaction and Qubit® 3.0 Fluorometer (Invitrogen, USA) was used to quantify the size of Library fragments. Sequencing was then performed on a Nanopore PromethION sequencer (Oxford Nanopore Technologies, UK) instrument on Nextomics. |
WGS |
GENOMIC |
RANDOM |
SINGLE
|
|
Processing |
|
Release date | 2024-02-26 |
Run |
Run accession |
Run data file information |
File name | File size (MB) |
CRR856414 |
CRR856414.fq.gz
|
38,351.65
|
CRR856415 |
CRR856415.fq.gz
|
14,538.59
|
CRR856416 |
CRR856416.fq.gz
|
36,860.13
|
CRR856417 |
CRR856417.fq.gz
|
33,464.32
|
CRR856418 |
CRR856418.fq.gz
|
22,471.98
|
CRR856419 |
CRR856419.fq.gz
|
30,465.46
|
CRR856420 |
CRR856420.fq.gz
|
34,243.82
|
CRR856421 |
CRR856421.fq.gz
|
20,198.73
|
CRR856422 |
CRR856422.fq.gz
|
40,321.05
|
CRR856423 |
CRR856423.fq.gz
|
52,014.93
|
CRR856424 |
CRR856424.fq.gz
|
58,555.77
|
CRR856425 |
CRR856425.fq.gz
|
54,359.31
|
CRR856426 |
CRR856426.fq.gz
|
54,047.3
|
|
Submitter | Dongmei Yin (yindm@126.com) |
Organization | Henan Agricultural University |
Date submitted | 2023-08-23 |