| 实验编号 | CRX772458 |
| 物种名称 | Arachis monticola |
| 标题 | Long-read sequencing of Arachis monticola |
| 项目编号 | PRJCA019219 |
| 样本编号 | SAMC3013710 |
| 测序平台 | OXFORD_NANOPORE PromethION |
| 建库信息 |
| 文库名称 |
文库构建方法 |
建库策略 |
样品来源 |
实验选择 |
文库布局 |
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A total amount of 2 µg DNA per sample was used as input material for the ONT library preparations. After the sample was qualified, size-select of long DNA fragments were performed using the BluePippin system (Sage Science, USA). Next, the ends of DNA fragments were repaired and A-ligation reaction were conducted with NEBNext Ultra II End Repair/dA-tailing Kit (Cat# E7546). The adapter in the LSK109 kit was used for further ligation reaction and Qubit® 3.0 Fluorometer (Invitrogen, USA) was used to quantify the size of Library fragments. Sequencing was then performed on a Nanopore PromethION sequencer (Oxford Nanopore Technologies, UK) instrument on Nextomics. |
WGS |
GENOMIC |
RANDOM |
SINGLE
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| 处理信息 |
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| 发布日期 | 2024-02-26 |
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| 测序反应 |
| Run编号 |
Run序列文件信息 |
| File name | File size (MB) |
|---|
| CRR856414 |
CRR856414.fq.gz
|
38,351.65
|
| CRR856415 |
CRR856415.fq.gz
|
14,538.59
|
| CRR856416 |
CRR856416.fq.gz
|
36,860.13
|
| CRR856417 |
CRR856417.fq.gz
|
33,464.32
|
| CRR856418 |
CRR856418.fq.gz
|
22,471.98
|
| CRR856419 |
CRR856419.fq.gz
|
30,465.46
|
| CRR856420 |
CRR856420.fq.gz
|
34,243.82
|
| CRR856421 |
CRR856421.fq.gz
|
20,198.73
|
| CRR856422 |
CRR856422.fq.gz
|
40,321.05
|
| CRR856423 |
CRR856423.fq.gz
|
52,014.93
|
| CRR856424 |
CRR856424.fq.gz
|
58,555.77
|
| CRR856425 |
CRR856425.fq.gz
|
54,359.31
|
| CRR856426 |
CRR856426.fq.gz
|
54,047.3
|
|
| 提交者 | Dongmei Yin (yindm@126.com) |
|---|
| 所属单位 | Henan Agricultural University |
|---|
| 提交日期 | 2023-08-23 |
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