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Experiment information
Accession CRX772458
Organism Arachis monticola
Title Long-read sequencing of Arachis monticola
BioProject PRJCA019219
BioSample SAMC3013710
Platform OXFORD_NANOPORE PromethION
Library
Library name Construction protocol Strategy Source Selection Layout
A total amount of 2 µg DNA per sample was used as input material for the ONT library preparations. After the sample was qualified, size-select of long DNA fragments were performed using the BluePippin system (Sage Science, USA). Next, the ends of DNA fragments were repaired and A-ligation reaction were conducted with NEBNext Ultra II End Repair/dA-tailing Kit (Cat# E7546). The adapter in the LSK109 kit was used for further ligation reaction and Qubit® 3.0 Fluorometer (Invitrogen, USA) was used to quantify the size of Library fragments. Sequencing was then performed on a Nanopore PromethION sequencer (Oxford Nanopore Technologies, UK) instrument on Nextomics. WGS GENOMIC RANDOM SINGLE
Processing
Release date2024-02-26
Run
Run accession Run data file information
File nameFile size (MB)
CRR856414 CRR856414.fq.gz 38,351.65
CRR856415 CRR856415.fq.gz 14,538.59
CRR856416 CRR856416.fq.gz 36,860.13
CRR856417 CRR856417.fq.gz 33,464.32
CRR856418 CRR856418.fq.gz 22,471.98
CRR856419 CRR856419.fq.gz 30,465.46
CRR856420 CRR856420.fq.gz 34,243.82
CRR856421 CRR856421.fq.gz 20,198.73
CRR856422 CRR856422.fq.gz 40,321.05
CRR856423 CRR856423.fq.gz 52,014.93
CRR856424 CRR856424.fq.gz 58,555.77
CRR856425 CRR856425.fq.gz 54,359.31
CRR856426 CRR856426.fq.gz 54,047.3
SubmitterDongmei Yin (yindm@126.com)
OrganizationHenan Agricultural University
Date submitted2023-08-23
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