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Experiment information
Accession CRX772459
Organism Arachis monticola
Title Short-read sequencing of Arachis monticola
BioProject PRJCA019219
BioSample SAMC3013710
Platform MGISEQ-2000
Library
Library name Construction protocol Strategy Source Selection Layout
1-1.5μg genomic DNA was randomly fragmented by Covaris, The fragmented DNA was selected by Agencourt AMPure XP-Medium kit to an average size of 200-400bp. The selected fragments were through end-repair, 3’ adenylated, adapters-ligation, PCR Amplifying and the products were recovered by the AxyPrep Mag PCR clean up Kit. The double stranded PCR products were heat denatured and circularized by the splint oligo sequence. The single strand circle DNA (ssCir DNA) were formatted as the final library and qualified by QC. The qualified libraries were sequenced on MGISEQ2000 platform. WGS GENOMIC PCR PAIRED
Processing Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Release date2024-02-26
Run
Run accession Run data file information
File nameFile size (MB)
CRR856427 CRR856427_f1.fq.gz
CRR856427_r2.fq.gz
85,058.57
87,091.8
CRR856428 CRR856428_f1.fq.gz
CRR856428_r2.fq.gz
14,199.36
14,716.55
SubmitterDongmei Yin (yindm@126.com)
OrganizationHenan Agricultural University
Date submitted2023-08-23
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Experiments(2)