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实验基本信息
实验编号 CRX772459
物种名称 Arachis monticola
标题 Short-read sequencing of Arachis monticola
项目编号 PRJCA019219
样本编号 SAMC3013710
测序平台 MGISEQ-2000
建库信息
文库名称 文库构建方法 建库策略 样品来源 实验选择 文库布局
1-1.5μg genomic DNA was randomly fragmented by Covaris, The fragmented DNA was selected by Agencourt AMPure XP-Medium kit to an average size of 200-400bp. The selected fragments were through end-repair, 3’ adenylated, adapters-ligation, PCR Amplifying and the products were recovered by the AxyPrep Mag PCR clean up Kit. The double stranded PCR products were heat denatured and circularized by the splint oligo sequence. The single strand circle DNA (ssCir DNA) were formatted as the final library and qualified by QC. The qualified libraries were sequenced on MGISEQ2000 platform. WGS GENOMIC PCR PAIRED
处理信息 Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
发布日期2024-02-26
测序反应
Run编号 Run序列文件信息
File nameFile size (MB)
CRR856427 CRR856427_f1.fq.gz
CRR856427_r2.fq.gz
85,058.57
87,091.8
CRR856428 CRR856428_f1.fq.gz
CRR856428_r2.fq.gz
14,199.36
14,716.55
提交者Dongmei Yin (yindm@126.com)
所属单位Henan Agricultural University
提交日期2023-08-23
关联实验
Experiments(2)