| Accession | CRX772460 |
| Organism | Arachis monticola |
| Title | Hi-C of Arachis monticola |
| BioProject | PRJCA019219 |
| BioSample | SAMC3013710 |
| Platform | MGISEQ-2000 |
| Library |
| Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
To anchor hybrid scaffolds onto the chromosome, genomic DNA was extracted for the Hi-C library from Arachis monticola. Then, we constructed the Hi-C library and obtained sequencing data via the MGI-2000 platform. In brief, freshly harvested leaves were cut into 2 cm pieces and vacuum infiltrated in nuclei isolation buffer supplemented with 2% formaldehyde. Crosslinking was stopped by adding glycine and additional vacuum infiltration. Fixed tissue was then grounded to powder before re-suspending in nuclei isolation buffer to obtain a suspension of nuclei. The purified nuclei were digested with 100 units of DpnII and marked by incubating with biotin-14-dCTP. Biotin-14-dCTP from non-ligated DNA ends was removed owing to the exonuclease activity of T4 DNA polymerase. The ligated DNA was sheared into 300-600 bp fragments, and then was blunt-end repaired and A-tailed, followed by purification through biotin-streptavidin-mediated pull down. Finally, the Hi-C libraries were quantified and sequenced using the MGI-2000 platform. |
Hi-C |
GENOMIC |
RANDOM |
PAIRED
|
|
| Processing |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
|
| Release date | 2024-02-26 |
|---|
| Run |
| Run accession |
Run data file information |
| File name | File size (MB) |
|---|
| CRR856429 |
CRR856429_f1.fq.gz
CRR856429_r2.fq.gz
|
45,279.91
48,978.84
|
| CRR856430 |
CRR856430_f1.fq.gz
CRR856430_r2.fq.gz
|
57,831.28
65,933.23
|
| CRR856431 |
CRR856431_f1.fq.gz
CRR856431_r2.fq.gz
|
7,755.39
8,113.79
|
|
| Submitter | Dongmei Yin (yindm@126.com) |
|---|
| Organization | Henan Agricultural University |
|---|
| Date submitted | 2023-08-23 |
|---|
