| Accession | CRX777318 |
| Organism | metagenome |
| Title | MALBAC.GY14-2 |
| BioProject | PRJCA019347 |
| BioSample | SAMC3036755 |
| Platform | Illumina NovaSeq 6000 |
| Library |
| Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
To mitigate the potential impact of preferential amplification methods on bacterial identification, for the amplification of genomic DNA, two widely-used methods, namely Multiple Displacement Amplification (MDA) and Multiple Annealing and Looping Based Amplification Cycles (MALBAC), were randomly applied in the biological replicates for each ciliate. The REPLI-g Single Cell Kit (Qiagen) based on MDA technology or the Single-Cell WGA Kit (Yikon, YK001A) based on MALBAC technology was used for genomic DNA amplification, following the manufacturer's guidelines. Subsequently, sequencing libraries were constructed using the TruSeq Nano DNA HT Sample Preparation Kit (Illumina). High-throughput sequencing was performed on the Illumina NovaSeq 6000 platform using PE150 sequencing chemistry. |
WGS |
GENOMIC |
RANDOM |
PAIRED
|
|
| Processing |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
|
| Release date | 2023-08-31 |
|---|
| Run |
| Run accession |
Run data file information |
| File name | File size (MB) |
|---|
| CRR861517 |
CRR861517_f1.fq.gz
CRR861517_r2.fq.gz
|
3,374.92
3,507.43
|
|
| Submitter | bing zhang (bingzhang_bio@qq.com) |
|---|
| Organization | Beijing Institutes of Life Science, Chinese Academy of Sciences |
|---|
| Date submitted | 2023-08-31 |
|---|
