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文库构建方法 |
建库策略 |
样品来源 |
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文库布局 |
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Total RNA was extracted from the tumor samples, and RNA integrity was assessed through agarose gel electrophoresis, while RNA purity was determined using Nanodrop. RNA quantity was accurately measured using Qubit. Following mRNA enrichment, the library construction process included mRNA fragmentation, strand-specific cDNA synthesis, adapter ligation, and other necessary steps. Ultimately, high-throughput sequencing was conducted. |
RNA-Seq |
TRANSCRIPTOMIC |
PolyA |
PAIRED
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