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Experiment information
Accession CRX824631
Organism Bacteria
Title Raw 16S rRNA gene sequencing data of S. putrefaciens-R. palustris co-culture without Fe redox communication cultured for 1 day
BioProject PRJCA020292
BioSample SAMC3093955
Platform Illumina MiSeq
Library
Library name Construction protocol Strategy Source Selection Layout
V3 and V4 regions of 16S rRNA were amplified by PCR with primers of 341F:5'-CCTA CGGG NGGC WGCA G-3' and 805R: 5'-GACT ACHV GGGT ATCT AATC C-3'. The amplified DNA was then processed for gene sequencing with Illumina MiSeq-PE250 system. QIIME2 was adopted to analyze the gene sequences and classify the operational taxonomic units (OTUs) on the basis of 97% sequence identity. AMPLICON OTHER PCR PAIRED
Processing Planned read length (bp) for mate 1: 250
Planned read length (bp) for mate 2: 250
Release date2023-10-07
Run
Run accession Run data file information
File nameFile size (MB)
CRR911465 CRR911465_f1.fastq.gz
CRR911465_r2.fastq.gz
8.01
8.4
SubmitterNa Chen (chenna0804@whu.edu.cn)
OrganizationWuhan University
Date submitted2023-10-07