Accession | CRX824633 |
Organism | Bacteria |
Title | Raw 16S rRNA gene sequencing data of S. putrefaciens-R. palustris co-culture without Fe redox communication cultured for 4 day |
BioProject | PRJCA020292 |
BioSample | SAMC3093957 |
Platform | Illumina MiSeq |
Library |
Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
V3 and V4 regions of 16S rRNA were amplified by PCR with primers of 341F:5'-CCTA CGGG NGGC WGCA G-3' and 805R: 5'-GACT ACHV GGGT ATCT AATC C-3'. The amplified DNA was then processed for gene sequencing with Illumina MiSeq-PE250 system. QIIME2 was adopted to analyze the gene sequences and classify the operational taxonomic units (OTUs) on the basis of 97% sequence identity. |
AMPLICON |
OTHER |
PCR |
PAIRED
|
|
Processing |
Planned read length (bp) for mate 1: 250
Planned read length (bp) for mate 2: 250
|
Release date | 2023-10-07 |
Run |
Run accession |
Run data file information |
File name | File size (MB) |
CRR911467 |
CRR911467_f1.fastq.gz
CRR911467_r2.fastq.gz
|
6.84
7.44
|
|
Submitter | Na Chen (chenna0804@whu.edu.cn) |
Organization | Wuhan University |
Date submitted | 2023-10-07 |