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| 文库名称 |
文库构建方法 |
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1000 ng of DNA per sample were end-repaired, and AMPure XP beads were used to purify DNA. Next, Each DNA was ligated with a unique barcode with NEB Quick Ligation Module. After the concentration was measured with Qubit Fluorometer, twelve or more barcoded DNA samples were pooled in equal mass ratios. Then, the pooled barcoded samples were ligated to a sequencing adaptor for nanopore sequencing using NEBNext Blunt/TA Ligase Master Mix . Using a MinION flow cells to prime and load the ligation library. |
OTHER |
METAGENOMIC |
unspecified |
SINGLE
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