| Accession | CRX844946 |
| Organism | metagenome |
| Title | AOMDSSFnKrasG12D6 |
| BioProject | PRJCA020969 |
| BioSample | SAMC3144412 |
| Platform | Illumina MiSeq |
| Library |
| Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
The total DNA was then extracted with the QIAamp DNA Mini kit (QIAGEN). The 16S rRNA high-throughput sequencing was performed by Realbio Genomics Institute (Shanghai, China) using the Illumina MiSeq. Variable regions V3-V4 on 16S rRNA genes of bacteria were amplified with forward primer F341 5’-ACTCCTACGGGRSGCAGCAG-3’ and reverse primer R806 5’-GGACTACVVGGGTATCTAATC-3’. The raw data were then subjected to a quality control procedure using UPARSE. The qualified reads were clustered to generate operational taxonomic units (OTUs) at the 97% similarity level using Usearch. |
AMPLICON |
GENOMIC |
PCR |
PAIRED
|
|
| Processing |
Planned read length (bp) for mate 1: 250
Planned read length (bp) for mate 2: 250
|
| Release date | 2023-12-18 |
|---|
| Run |
| Run accession |
Run data file information |
| File name | File size (MB) |
|---|
| CRR933225 |
CRR933225_f1.fastq.gz
CRR933225_r2.fastq.gz
|
2.88
3.95
|
|
| Submitter | Huanlong Qin (qinhuanlong@tongji.edu.cn) |
|---|
| Organization | Shanghai Tenth Peolple's Hospital |
|---|
| Date submitted | 2023-11-01 |
|---|
