| 实验编号 | CRX873396 |
| 物种名称 | Phoenix dactylifera |
| 标题 | trcp96h1 |
| 项目编号 | PRJCA021696 |
| 样本编号 | SAMC3212353 |
| 测序平台 | Illumina NovaSeq 6000 |
| 建库信息 |
| 文库名称 |
文库构建方法 |
建库策略 |
样品来源 |
实验选择 |
文库布局 |
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The method for synthesizing the first strand of cDNA by reverse transcription is the same as the common library building method of NEB. The difference is that when synthesizing the second strand, dTTP in dNTPs is replaced by dUTP, and then the cDNA end repair, A-tail addition, sequencing joint connection and length screening are also carried out. Then, USER enzyme was used to degrade the second strand of cDNA containing U, and PCR amplification was performed to obtain the library. |
ncRNA-Seq |
OTHER |
PCR |
PAIRED
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|
| 处理信息 |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
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| 发布日期 | 2024-01-31 |
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| 测序反应 |
| Run编号 |
Run序列文件信息 |
| File name | File size (MB) |
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| CRR962480 |
CRR962480_f1.fq.gz
CRR962480_r2.fq.gz
|
2,907.55
3,058.2
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| 提交者 | Zhongliang Xu (xuzhongliangcatas@163.com) |
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| 所属单位 | Chinese Academy of Tropical Agriculture Sciences |
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| 提交日期 | 2023-12-05 |
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